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ABC-ELISA-A new method suitable for large-scale serological assessment of malaria Sato, Kumiko Yamaguchi, Haruyasu Kano, Shigeyuki Suzuki, Mamoru ABC-ELISA ELISA Malaria serological test application/pdf Departmental Bulletin Paper The authors developed a new practical method for the measurement of malaria antibodies which met the requirements needed for large-scale malaria assessment in the tropics. The new method, ABC-ELISA has used the avidin biotin peroxidase complex system for the amplification of the ordinary ELISA substrate reactions in malaria serological tests. The ABC-ELISA was more sensitive than the ordinary ELISA through the use of the ABC system. This allowed the resulting color spots on the dried plate wells to be read clearly with the naked eye. The method was able to differentiate well malaria patients with recent past history from subjects. The sera from malaria patients were examined using three kinds of sero-immunological methods: ABC-ELISA, fluorescence enzyme linked immunosorbent assay (fluorescence-ELISA), and indirect fluorescent antibody technique (IFAT). An excellent correlation between the ABC-ELISA and IFAT titers was obtained; a correlation coefficient being 0.923. ABC-ELISA titers also correlated well with fluorescence-ELISA titers; the correlation coefficient being 0.962. The lyophilization of the malaria antigen preserved the antigen activity without inducing any nonspecific reaction. These results suggest that the ABC-ELISA is useful for assessing malaria survey in tropical endemic areas, because the test can be carried out without using major electrical equipment. 群馬大学医療技術短期大学部 1992-03-31 2017-03-27 eng departmental bulletin paper VoR http://hdl.handle.net/10087/1983 https://gunma-u.repo.nii.ac.jp/records/1580 AN00068340 0389-7540 群馬大学医療技術短期大学部紀要 12 133 141 https://gunma-u.repo.nii.ac.jp/record/1580/files/KJ00004436245.pdf application/pdf 452.6 kB 2020-11-18