2026-03-14T03:05:46Z https://gunma-u.repo.nii.ac.jp/oai

oai:gunma-u.repo.nii.ac.jp:00001880 2023-06-19T13:35:53Z 384:404:405:416

Neuronal expression of c-Fos protein in the brain after intraperitoneal injection of leptin in Wistar rats TAKAYAMA, Kiyoshige KAWATA, Haruka TAKAHARA, Ryohta TOMITA, Kazuhide Brain Central nervous system c-Fos Leptin Neurons application/pdf Departmental Bulletin Paper Leptin has been recognized to be an important neuroendocrine signal in the\nregulation of food intake and energy balance. We aimed to survey central neurons that might be\nactivated after peripheral administration of leptin, by examining the distribution of neurons\nexpressing c-Fos protein. Leptin dissolved at a dose of 500 μg/kg in physiological saline was\nintraperitoneally injected in Wistar rats. One and a half hours after the injection, rats were\ntranscardially perfused with saline and fixed with fixatives. The brain was removed and\nsectioned at 40 μm in thickness. Every fourth section was treated with anti-c-Fos antiserum,\nand c-Fos protein was immunohistochemically stained using the avidin-biotin complex method.\nControl rats were injected with saline solution, and brain sections were processed similarly as\ndescribed above. It was found that leptin injected intraperitoneally induced the neuronal\nexpression of c-Fos protein in several nuclei throughout the brain. In the central nucleus\namygdala, ventromedial nucleus of hypothalamus, periaqueductal gray matter, lateral\nparabrachial nucleus, and the solitary tract nucleus, numbers of neurons expressing c-Fos\nprotein were much more in the test experiments than those in the control experiments.\nIntraperitoneally injected leptin was found to stimulate central neurons that may play some\nroles in the regulation of such as a food intake. 群馬大学医学部保健学科 2008-03 2017-03-27 eng departmental bulletin paper VoR http://hdl.handle.net/10087/5265 https://gunma-u.repo.nii.ac.jp/records/1880 AA11260213 1343-4179 群馬保健学紀要 28 1 8 https://gunma-u.repo.nii.ac.jp/record/1880/files/HKJ28-001-008.pdf application/pdf 187.5 kB 2020-11-18