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Genome-wide profiling of promoter methylation in human
http://hdl.handle.net/10087/2967
http://hdl.handle.net/10087/29676df054e2-9ab4-4c17-9515-0f28ca29be87
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Oncogene25_21_3059.pdf (858.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-03-28 | |||||
| タイトル | ||||||
| タイトル | Genome-wide profiling of promoter methylation in human | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DNA methylation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | genome-wide profiling | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | epigenetics | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microarray | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Hatada, Izuho
× Hatada, Izuho× Fukasawa, Masayuki× Kimura, Mika× Morita, Sumiyo× Yamada, Kazuo× Yoshikawa, Takeo× Yamanaka, Sumitaka× Endo, Chiaki× Sakurada, Akira× Sato, Masami× Kondo, Takashi× Horii, Akira× Ushijima, Toshikazu× Sasaki, Hiroyuki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA methylation in the promoter region of a gene is associated with a loss of that gene's expression and plays an important role in gene silencing. The inactivation of tumor-suppressor genes by aberrant methylation in the promoter region is well recognized in carcinogenesis. However, there has been little study in this area when it comes to genome-wide profiling of the promoter methylation. Here, we developed a genome-wide profiling method called Microarray-based Integrated Analysis of Methylation by Isoschizomers to analyse the DNA methylation of promoter regions of 8091 human genes. With this method, resistance to both the methylation-sensitive restriction enzyme HpaII and the methylation-insensitive isoschizomer MspI was compared between samples by using a microarray with promoter regions of the 8091 genes. The reliability of the difference in HpaII resistance was judged using the difference in MspI resistance. We demonstrated the utility of this method by finding epigenetic mutations in cancer. Aberrant hypermethylation is known to inactivate tumour suppressor genes. Using this method, we found that frequency of the aberrant promoter hypermethylation in cancer is higher than previously hypothesized. Aberrant hypomethylation is known to induce activation of oncogenes in cancer. Genome-wide analysis of hypomethylated promoter sequences in cancer demonstrated low CG/GC ratio of these sequences, suggesting that CpG-poor genes are sensitive to demethylation activity in cancer. | |||||
| 書誌情報 |
Oncogene 巻 25, 号 21, p. 3059-3064, 発行日 2006-05-18 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0950-9232 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10687380 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1038/sj.onc.1209331 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 出版者 | ||||||
| 出版者 | Nature Publishing Group | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
| 関係URI | ||||||
| 関連名称 | http://www.nature.com/npg/ | |||||
| 更新日 | ||||||
| 日付 | 2017-03-27 | |||||
| 日付タイプ | Created | |||||
