{"created":"2023-06-19T08:35:15.852890+00:00","id":1336,"links":{},"metadata":{"_buckets":{"deposit":"1973ac75-1be7-4208-b289-5790f38dfa41"},"_deposit":{"created_by":2,"id":"1336","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1336"},"status":"published"},"_oai":{"id":"oai:gunma-u.repo.nii.ac.jp:00001336","sets":["384:385:386:387"]},"author_link":["1854","1855","1856"],"item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Studies on Globin Specific Protease of Chlonorchis Sinenis"}]},"item_5_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1981-03-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"82","bibliographicPageStart":"73","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"群馬大学医療技術短期大学部紀要"}]}]},"item_5_date_61":{"attribute_name":"更新日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017-03-27","subitem_date_issued_type":"Created"}]},"item_5_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_description_39":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Departmental Bulletin Paper","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"肝吸虫の粗抽出液について、セファデックスG-200を用いたゲル濾過、およびDEAE-セルローズカラムクロマトグラフィーを順次行って、特異的グロビン分解酵素の精製を試みた。その結果、溶出条件の異なる3分画に酵素活性を認めたので、それらの分画、Cl1,Cl2,Cl3について、酵素学的性状を検討した。1.各分画の酵素はいずれも、反応時間に比例して活性が増加し、かなり安定な酵素と考えられた。また、至適pHはCl1がpH3.5～3.6,Cl2,Cl3がpH3.8～3.9であった。2.各分画の酵素のKm値はそれぞれ、Cl1,Km≒0.11mM,Cl2,Km≒0.16mM,Cl3,Km≒0.046mMであった。3.これらの酵素はいずれも、Hbやミオグロビンなどを特異的に水解したが、Cl2は他の酵素に比較して卵白アルブミンなどを多少水解し、基質特異性の低い傾向がみられた。4.いずれの酵素も、N-α-トシル-L-リシルークロロメタン、ジエチルジチオカルバメイト、モノヨード酢酸によって阻害された。","subitem_description_type":"Abstract"}]},"item_5_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"群馬大学医療技術短期大学部"}]},"item_5_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11389413","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0389-7540","subitem_source_identifier_type":"ISSN"}]},"item_5_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 久美子"}],"nameIdentifiers":[{"nameIdentifier":"1854","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"武井, 一利"}],"nameIdentifiers":[{"nameIdentifier":"1855","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小川, 純子"}],"nameIdentifiers":[{"nameIdentifier":"1856","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-18"}],"displaytype":"detail","filename":"KJ00004436645.pdf","filesize":[{"value":"483.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004436645.pdf","url":"https://gunma-u.repo.nii.ac.jp/record/1336/files/KJ00004436645.pdf"},"version_id":"b52fcc70-67b0-42cd-9fcb-52a6b23e2d50"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"肝吸虫Clonorchis sinensisの特異的グロビン分解酵素の研究 : 酵素の精製と性状について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"肝吸虫Clonorchis sinensisの特異的グロビン分解酵素の研究 : 酵素の精製と性状について"}]},"item_type_id":"5","owner":"2","path":["387"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-17"},"publish_date":"2007-12-17","publish_status":"0","recid":"1336","relation_version_is_last":true,"title":["肝吸虫Clonorchis sinensisの特異的グロビン分解酵素の研究 : 酵素の精製と性状について"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-06-19T13:48:32.865419+00:00"}