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Effect of Temperature on Polyacrylamide Gel Electrophoresis and Chromatography of Histones and Protamines
http://hdl.handle.net/10087/1800
http://hdl.handle.net/10087/1800497809f3-dad2-4ffb-9e77-757247b891d1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004436648.pdf (611.3 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||
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| 公開日 | 2007-12-17 | |||||||
| タイトル | ||||||||
| タイトル | Effect of Temperature on Polyacrylamide Gel Electrophoresis and Chromatography of Histones and Protamines | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 著者 |
Hamana, Koei
× Hamana, Koei
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Polyacrylamide gel electrophoresis or chromatography of histories (H1, H5, H2A, H2B, H3 and H4) and protamines (salmine and clupeine) in 0.9M acetic acid or 0.01M HC1, respectively, was carried out at various temperatures. A retardation of either electrophoretic migration or chromatographic elution of the proteins was observed at lower temperatures. H1 and H5 histones exhibited the least extent of retardation. The retardation with lowering temperature was also observed in the presence of 0.02-0.05M NaCl but not in the presence of 5-6.25M urea. The extent of retardation in polyacrylamide gel chromatography with lowering temperature increased with proteins of higher arginine contents. The fact was explained as that ionic interactions between guanido groups of arginine residues in the proteins and carboxyl groups of polyacrylamide are strengthened at lower gel temperatures. | |||||||
| 書誌情報 |
群馬大学医療技術短期大学部紀要 巻 1, p. 106-119, 発行日 1981-03-10 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0389-7540 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA11389413 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版者 | ||||||||
| 出版者 | 群馬大学医療技術短期大学部 | |||||||
| 資源タイプ | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Departmental Bulletin Paper | |||||||
| 更新日 | ||||||||
| 日付 | 2017-03-27 | |||||||
| 日付タイプ | Created | |||||||
