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Gel Electrophoresis, Gel Chromatography and Sedimentation Analysis of Histones and Protamines in the Presence of Nonionic Surfactants
http://hdl.handle.net/10087/1863
http://hdl.handle.net/10087/186346d9fe76-173d-4153-84d3-94ab9b1b7855
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-12-17 | |||||
タイトル | ||||||
タイトル | Gel Electrophoresis, Gel Chromatography and Sedimentation Analysis of Histones and Protamines in the Presence of Nonionic Surfactants | |||||
言語 | ||||||
言語 | eng | |||||
著者 |
Hamana, Koei
× Hamana, Koei |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Histones and protamines behaved as a monomeric form in 0.9M acetic acid. When gel electrophoresis was carried out in 0.9M acetic acid, the mobilities of calf H2A, H2B, H3 and H4 histones were decreased proportionaly to the helix content of the histones but the mobilities of calf H1 histone and fish protamines were not decreased by the addition of 6mM nonionic surfactant into the gel. Degree of the decreasing on the mobilities of four histones was also proportional to the molecular weight of the added nonionic surfactant. Structure of hydrophobic group of the surfactants was more affective than polyoxyethylene chain length of the surfactants. H2B, H3 and H4 histones aggregated in 0.05-0.1M sodium phosphate buffer, pH 7.0, while H1 and H2A histones behaved as a monomeric form. Nonionic surfactants bound to H2A histone but did not to H1 histone and to the aggregates of other histones in the buffer. | |||||
書誌情報 |
群馬大学医療技術短期大学部紀要 巻 2, p. 165-177, 発行日 1982-03-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0389-7540 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11389413 | |||||
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内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
出版者 | ||||||
出版者 | 群馬大学医療技術短期大学部 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Departmental Bulletin Paper | |||||
更新日 | ||||||
日付 | 2017-03-27 | |||||
日付タイプ | Created |