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ニワトリ肝細胞クロマチンの蛋白質成分とエストラジオール結合親和性
http://hdl.handle.net/10087/2076
http://hdl.handle.net/10087/2076118ff84d-5118-4952-908f-80c2347c610e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004436076.pdf (603.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-12-20 | |||||
| タイトル | ||||||
| タイトル | ニワトリ肝細胞クロマチンの蛋白質成分とエストラジオール結合親和性 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| その他のタイトル | ||||||
| その他のタイトル | Protein Components and Estradiol-Binding Affinity of Chicken Liver Chromatin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chromain | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Estradiol | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chicken Liver | |||||
| 著者 |
浜名, 康栄
× 浜名, 康栄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nuclei and chromatins were isolated from the livers of egg-laying hens, roosters, and chicken embryos. The chromatin proteins were analyzed by urea-SDS gel electrophoresis. Major nonhistone proteins, NHP-1, -2, -3, -4, -5, were rich in the liver chromatin of laying hen. Higher histone content was found in the rooster liver chromatin. The loss of H1 histones during the preparation of laying hen chromatin suggests the loosely binding of H1 histone in the chromatin. A nonhistone protein, NHP-1, was found only in the mature female and male chicken liver chromatins. This component was not detected in the embryonic liver chromatins and chicken erythrocyte chromatins. Occurrence of an another nonhistone protein, NHP-2, was specific for laying hen liver chromatin. NHP-1 and NHP-4 (HMG protein) were preferentially released from the chromatin by DNase I digestion. Cytoplasmic estrogen receptors were found in both laying hen and rooster livers. The isolated nuclei and chromatin from laying hen liver showed a high binding affinity for free ^3H-estradiol. However, ^3H-estradiol-receptor complex faild to bind significantlly to the isolated liver nuclei of laying hen. It is suggested that H1 histone was replaced with NHP-1 and/or NHP-2 on the active regions of laying hen liver chromatin in which vitellogenin genes are activating, NHP-1 is essential for the induction of the gene expression, and NHP-2 localize on the acceptor sites for estrogen-receptor complex in the chromatin. | |||||
| 書誌情報 |
群馬大学医療技術短期大学部紀要 巻 8, p. 65-73, 発行日 1988-02-29 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0389-7540 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00068340 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版者 | ||||||
| 出版者 | 群馬大学医療技術短期大学部 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Departmental Bulletin Paper | |||||
| 更新日 | ||||||
| 日付 | 2017-03-27 | |||||
| 日付タイプ | Created | |||||
