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Tetrahymenaの細胞周期におけるポリアミン含量の変動とポリアミンによるクロマチン蛋白質の解離
http://hdl.handle.net/10087/1954
http://hdl.handle.net/10087/19543e3448fe-b98d-4b6a-a6df-ba724bec80eb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004436114.pdf (817.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2007-12-20 | |||||||||
| タイトル | ||||||||||
| タイトル | Tetrahymenaの細胞周期におけるポリアミン含量の変動とポリアミンによるクロマチン蛋白質の解離 | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | Changes of Polyamine Levels during Cell Cycle and Dissociation of Chromatin Proteins by Plyamines in Tetrahymena | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Polyamine | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Chromatin | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Tetrahymena | |||||||||
| 著者 |
浜名, 康栄
× 浜名, 康栄
× 松崎, 茂
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | A cilliate protozoon, Tetrahymena contains putrescine and spermidine as major polyamines. In subcellular distribution, putrescine occurred in high concentrations in cytosol and spermidine in nuclear and ribosomal fractions. Cellular polyamine levels increased during the logarithmic growth phase and then decreased in the stationary phase. Polyamine levels were lowest in the starved cells in the G_0 stage and then, with re-feeding, increased during the G_1 and S stages. With heat treatment, the polyamine levels started to rise in the G_1 stage in the synchronized cell division cycle. These findings show that polyamine synthesis was initiated prior to DNA and RNA syntheses. Isolated macronuclei from Tetrahymena retained rigid forms in the presence of 4mM spermidine, while the nuclei were transformed into chromatin fibers in the absence of polyamines. However, LG-1 (HMG) Protein was selectively extracted from the nuclei with O.1mM salmine (a protamine), 1mM spermine, 16mM putrescine, or 4mM spermidine. These concentrations are closely correlated with their positive chrge per molecule. Changes of cellular polyamine levels during growth or cell cycles may affect chromatin structure and function in eukaryotes. | |||||||||
| 書誌情報 |
群馬大学医療技術短期大学部紀要 巻 9, p. 33-38, 発行日 1989-03-31 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0389-7540 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AN00068340 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版者 | ||||||||||
| 出版者 | 群馬大学医療技術短期大学部 | |||||||||
| 資源タイプ | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Departmental Bulletin Paper | |||||||||
| 更新日 | ||||||||||
| 日付 | 2017-03-27 | |||||||||
| 日付タイプ | Created | |||||||||
