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出芽酵母Saccharomycesおよび分裂酵母Schizosaccharomycesのポリアミン合成に対する各種阻害剤の影響
http://hdl.handle.net/10087/2183
http://hdl.handle.net/10087/2183aff2ebec-da7b-472a-9ee3-6dd759a9c952
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004436193.pdf (364.7 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2007-12-21 | |||||||||
| タイトル | ||||||||||
| タイトル | 出芽酵母Saccharomycesおよび分裂酵母Schizosaccharomycesのポリアミン合成に対する各種阻害剤の影響 | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | Effects of Various Inhibitors on Polyamine Synthesis of Budding Yeast Saccharomyces and Fission Yeast Schizosaccharomyces | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Polyamine | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Inhibitor | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Yeast | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Saccharomyces | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Schizosaccharomyces | |||||||||
| 著者 |
浜名, 康栄
× 浜名, 康栄
× 松崎, 茂
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | We studied the effects of 10 drugs known to be inhibitors of ornithine decarboxylase (ODC), S-adenosyl-methionine decarboxylase (SAMDC), spermidine (Spd) synthase or spermine (Spm) synthase on the polyamine synthesis of the budding yeast Saccharomyces cerevisiae and the fission yeast Shizosaccharomyces pombe. Cellular polyamines were analyzed by high-performance liquid chromatography after culture in the absence or presence of the inhibitors. α-Difluoromethylornithine (DFMO), methylacetylenicputrescine (MAP) and α-hydrazinoornithine (HOrn), at 1-10mM, lowered putrescine (Put) levels in both yeasts, showing inhibition of the ODC activity. Trans-1,4-diamino-2-butene (DAB) at a concentration of 5mM decreased Put levels. The decrease in Spd and Spm levels suggests inhibition not only of ODC activity but also of Spd synthase and Spm synthase activities by DAB. At 5-10mM, 2-mercapto-ethylamine (MEA) and 3-methylthiopropylamine (MTPA) decreased Spd and Spm levels in each yeast. Put levels were rather increased by these two drugs, although the magnitude of Put increase was not the same between S. cerevisiae and S. pombe. Methylglyoxal-bis (guanylhydrazone) (MGBG), at 1-5mM, decreased Spd and Spm levels, suggesting inhibition of SAMDC activity as in mammalian and protozoan cells. Addition of 5-10mM of cyclohexylamine (CHA), 4-methylcyclohexylamine (MCHA) or N-(3-aminopropyl)cyclohexylamine (APCHA) resulted in decreases in cellular Spd and Spm levels, of both yeasts. CHA and MCHA appeared to preferentially inhibit Spd synthase activity, while APCHA specifically supressed Spm synthase activity. An increase in Put levels induced by the three drugs was observed in S. pombe but not in S. cerevisiae. It was suggested that the inhibitors are significantly effective against the polyamine synthesis of the yeasts, S. cerevisiae and S. pombe, as in mammalian and protozoan cells. However, there are some differences in sensitivity of these yeasts to the inhibitors tested. | |||||||||
| 書誌情報 |
群馬大学医療技術短期大学部紀要 巻 11, p. 39-44, 発行日 1991-03-29 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0389-7540 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AN00068340 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版者 | ||||||||||
| 出版者 | 群馬大学医療技術短期大学部 | |||||||||
| 資源タイプ | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Departmental Bulletin Paper | |||||||||
| 更新日 | ||||||||||
| 日付 | 2017-03-27 | |||||||||
| 日付タイプ | Created | |||||||||
