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ABC-ELISA-A new method suitable for large-scale serological assessment of malaria
http://hdl.handle.net/10087/1983
http://hdl.handle.net/10087/19836b026f3f-7527-4cd1-a94f-fd11d117d029
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004436245.pdf (452.6 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2007-12-20 | |||||
| タイトル | ||||||
| タイトル | ABC-ELISA-A new method suitable for large-scale serological assessment of malaria | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ABC-ELISA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ELISA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Malaria serological test | |||||
| 著者 |
Sato, Kumiko
× Sato, Kumiko× Yamaguchi, Haruyasu× Kano, Shigeyuki× Suzuki, Mamoru |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The authors developed a new practical method for the measurement of malaria antibodies which met the requirements needed for large-scale malaria assessment in the tropics. The new method, ABC-ELISA has used the avidin biotin peroxidase complex system for the amplification of the ordinary ELISA substrate reactions in malaria serological tests. The ABC-ELISA was more sensitive than the ordinary ELISA through the use of the ABC system. This allowed the resulting color spots on the dried plate wells to be read clearly with the naked eye. The method was able to differentiate well malaria patients with recent past history from subjects. The sera from malaria patients were examined using three kinds of sero-immunological methods: ABC-ELISA, fluorescence enzyme linked immunosorbent assay (fluorescence-ELISA), and indirect fluorescent antibody technique (IFAT). An excellent correlation between the ABC-ELISA and IFAT titers was obtained; a correlation coefficient being 0.923. ABC-ELISA titers also correlated well with fluorescence-ELISA titers; the correlation coefficient being 0.962. The lyophilization of the malaria antigen preserved the antigen activity without inducing any nonspecific reaction. These results suggest that the ABC-ELISA is useful for assessing malaria survey in tropical endemic areas, because the test can be carried out without using major electrical equipment. | |||||
| 書誌情報 |
群馬大学医療技術短期大学部紀要 巻 12, p. 133-141, 発行日 1992-03-31 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0389-7540 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00068340 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版者 | ||||||
| 出版者 | 群馬大学医療技術短期大学部 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Departmental Bulletin Paper | |||||
| 更新日 | ||||||
| 日付 | 2017-03-27 | |||||
| 日付タイプ | Created | |||||
