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A Development of a Computer Program for Normalization of the Test Results by Fluorescence Enzyme-linked Immunosorbent Assay for Specific IgE Antibody
http://hdl.handle.net/10087/2003
http://hdl.handle.net/10087/20039631a75c-9d94-4cdf-aed5-d56e36e757e5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004436283.pdf (450.6 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-12-20 | |||||
| タイトル | ||||||
| タイトル | A Development of a Computer Program for Normalization of the Test Results by Fluorescence Enzyme-linked Immunosorbent Assay for Specific IgE Antibody | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ELISA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fluorescence-ELISA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | specific IgE | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | normalization | |||||
| 著者 |
Sato, Kumiko
× Sato, Kumiko× Iida, Yoshihiro× Kanda, Kiyoko× Kano, Shigeyuki× Hida, Milton M.× Nakazawa, Tsugio |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Fluorescence enzyme-linked immunosorbent assay (fluorescence-ELISA) is used to measure antigen specific IgE antibodies of allergic and parasitic diseases. However, the data obtained from it are often affected by varying conditions. We tried to normalize relative fluorescence unit (RFU) value from fluorescence-ELISA, which was measured under various conditions, using a computer program. From the curve of the RFU value obtained from the reference serum at 2 times serial dilution, the modified exponential function, y=a+be^<-cx>, was applied to a regression curve to which data points were fitted. We prepared a microcomputer program to satisfy this algebraic equation, and carried out some simulation tests in order to demonstrate the viability of this program. It was confirmed that there was no significant deviation from the data points from the regression curve. Also the modified exponential function was good enough for quantitative analysis of RFU value obtained from fluorescence ELISA under varying conditions. The function well normalized the decreases in results of individual data obtained from various dilution of -D-galactosidase conjugated anti-human IgE antibodies. | |||||
| 書誌情報 |
群馬大学医療技術短期大学部紀要 巻 13, p. 111-119, 発行日 1993-03-31 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0389-7540 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00068340 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版者 | ||||||
| 出版者 | 群馬大学医療技術短期大学部 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Departmental Bulletin Paper | |||||
| 更新日 | ||||||
| 日付 | 2017-03-27 | |||||
| 日付タイプ | Created | |||||
