Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2008-10-05 |
タイトル |
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タイトル |
Viscoelastic and light scattering studies on thermally induced sol to gel phase transition in fish myosin solutions |
言語 |
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言語 |
eng |
キーワード |
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主題Scheme |
Other |
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主題 |
fish myosin |
キーワード |
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主題Scheme |
Other |
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主題 |
thermally induced gelation |
キーワード |
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主題Scheme |
Other |
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主題 |
viscoelasticity |
キーワード |
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主題Scheme |
Other |
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主題 |
dynamic light scattering |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
著者 |
Sawa, Kouchi
Kondo, Shingo
Ooi, Kazuko
Ichikawa, Hisashi
Dobashi, Toshiaki
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抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Viscoelastic (VE) and dynamic light scattering (DLS) analyses of fish (white croaker) myosin solutions were performed at myosin concentrations of 30mg/ml for VE and 0.1mg/ml for DLS at 0.6 MKCl and pH 7.0 to clarify thermally induced gelation. The hydrodynamic radius Rh considerably decreased around 30-35oC. The shear modulus G was constant below 25oC and increased by incubating the sample at 30oC. G further increased as the temperature of the incubated sample decreased. The curves of G vs. T for different time courses showed a sharp peak around 35oC and a moderate peak around 60oC in the heating process, while a stepwise increase in G was observed around 30oC in the cooling process when the temperature was elevated to not more than 60oC. No distinct stepwise change was observed once the temperature of the sample exceeded 60oC. The absolute value of G strongly depended on the maximum elevated temperature and the incubation time at that temperature. The corresponding behavior of the viscosity h was observed for each time course. Based on these results, the mechanism of thermally induced gelation of myosin solutions is discussed in view of S-S bridge formation in the head and tail portions and unwinding/rewinding of coiled-coil a-helices in the tail portion. |
書誌情報 |
Biopolymers
巻 69,
号 4,
p. 498-507,
発行日 2003-08
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ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-3525 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00566142 |
PubMed番号 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
PMID |
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関連識別子 |
12879495 |
DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
info:doi/info:doi/10.1002/bip.10388 |
フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
出版者 |
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出版者 |
Wiley |
資源タイプ |
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内容記述タイプ |
Other |
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内容記述 |
Journal Article |
更新日 |
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日付 |
2017-03-27 |
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日付タイプ |
Created |