| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2007-03-29 |
| タイトル |
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|
タイトル |
Systematic immunohistochemical profiling of 378 brain tumors with 37 antibodies using tissue microarray technology |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
brain tumors |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
tissue microarray |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
immunohistochemistry |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ikota, Hayato
Kinjo, Sawako
Yokoo, Hideaki
Nakazato, Yoichi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We performed a systematic immunohistochemical study on 378 brain tumors using 37 antibodies and tissue microarray (TMA) technology. The aim of this study was to find new diagnostic biomarkers using antibodies established in our laboratory. Our TMA consisted of a grid of 1.5-mm cores that were extracted from individual donor blocks. Staining for each antibody was scored using a three-point system. We used hierarchical clustering analysis to interpret these data, which resulted in separation of all the brain tumors into seven groups. Although there were some exceptions, cases with the same histological diagnosis were generally grouped together. We then carried out statistical analyses to find the most useful antibodies for grouping of brain tumors. Ten antibodies [glial fibrillary acidic protein (GFAP), Olig2, vimentin, epithelial membrane antigen (EMA), cytokeratin (AE1/AE3), alpha-internexin, nestin, pinealocytes PP5, aquaporin-4 (AQP4) M13d and AQP4M13e] discriminated between astrocytomas and oligodendroglial tumors. Six antibodies [EMA, AE1/AE3, TUJ1, nestin, neurofilament protein-MH (NF-MH) and perivascular cells GP-1] showed significant differences between high-grade and low-grade gliomas. Our data have revealed new antibodies with potential diagnostic utility (Olig2, PP5, GP-1) and demonstrate that TMA technology is highly useful for evaluating newly established antibodies in brain-tumor research. |
| 内容記述 |
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内容記述タイプ |
Other |
|
内容記述 |
The original publication is available at www.springerlink.com |
| 書誌情報 |
Acta Neuropathologica
巻 111,
号 5,
p. 475-482,
発行日 2006-05
|
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00508984 |
| PubMed番号 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
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関連識別子 |
16598485 |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
info:doi/10.1007/s00401-006-0060-1 |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 出版者 |
|
|
出版者 |
Springer Science + Business Media |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Journal Article |
| 関係URI |
|
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|
関連名称 |
http://www.springerlink.com/content/100394/ |
| 更新日 |
|
|
日付 |
2017-03-27 |
|
日付タイプ |
Created |
AN111_5_475.pdf (872.3 kB)
