{"created":"2023-06-19T08:37:59.354201+00:00","id":4745,"links":{},"metadata":{"_buckets":{"deposit":"33f47e55-ed45-4661-9e01-059030e0d425"},"_deposit":{"created_by":2,"id":"4745","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4745"},"status":"published"},"_oai":{"id":"oai:gunma-u.repo.nii.ac.jp:00004745","sets":["719:720:737:738"]},"author_link":["12400","12398","12394","12397","12395","12396","12399","12401"],"item_6_alternative_title_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Three cases of acanthamoeba keratitis : Detection of protozoa by ordinal cytological preparation of corneal scrapes"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"52","bibliographicPageStart":"48","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"日本臨床細胞学会雑誌"}]}]},"item_6_date_61":{"attribute_name":"更新日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017-03-27","subitem_date_issued_type":"Created"}]},"item_6_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_6_description_39":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"背景: アカントアメーバ角膜炎(Acanthamoeba keratitis)はコンタクトレンズ装着者に増加傾向がみられる.原因は淡水,土壌等に生息しているアカントアメーバ(Acanthamoeba)の感染によるもので,角膜擦過細胞診標本にて嚢子(cyst),培養検査にて原虫の嚢子(cyst),栄養体(trophozoite)を検出できた3例を経験したので報告した.症例: 角膜擦過細胞診で原虫の嚢子が確認できた.パパニコロウ染色ではライトグリーンにより嚢子壁が染色され,内部は茶褐色あるいは不染性であった.PAS染色では嚢子壁陽性,嚢子内部は暗赤色を呈していた.ギムザ染色では嚢子壁が青紫色に好染した.また,培養により得られた塗抹標本に蛍光染色を施し,蛍光を発するアカントアメーバの嚢子を検出できた.結論: アカントアメーバ角膜炎はアカントアメーバ原虫を検出し,早期に治療することが重要である.検出方法には病巣擦過物のパーカーインクKOH法による直接鏡検あるいは培養が代表的な検査方法であるが,角膜擦過細胞診標本でパパニコロウ染色,PAS染色,ギムザ染色標本の鏡検によるアカントアメーバ原虫の検出は簡便であり,早期に治療へ結びつくことができるため,有用な検査方法である.\\n\\nBackground: Acanthamoeba, a protozoan living in fresh water or soil, may infect the human cornea and provoke inflammation. Cases of acanthamoeba keratitis are increasing among contact lens users. We report three cases in which acanthamobea cysts were detected by ordinal cytological corneal scraping. Cases: All patients used contact lenses. Cytological examination of corneal scraping showed protozoan cysts. Papanicolaou staining showed the cyst wall as light green whereas the cyst interior stained as brown or unstained. In periodic acid Schiff staining, the cyst wall stained red-pink and the interior dark red. Gimsa staining showed the cyst wall as blue-violet. We judged the protozoa to be acanthamoeba as later confirmed by fluorescent staining applied to the cell culture of corneal scraping. Conclusion: To avoid serious complications in acanthamoeba keratitis, it is critical to detect causative organisms promptly. Optical detection by Parker Inke and KOH and cell culture of corneal scraping are conventional for acanthamoeba detection, but cytological preparation using Papalicolaou, PAS, and Gimsa staining is more convenient and rapid for early medical treatment of acanthamoeba keratitis.","subitem_description_type":"Abstract"}]},"item_6_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本臨床細胞学会"}]},"item_6_relation_43":{"attribute_name":"異版である","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://ci.nii.ac.jp/naid/110006571296","subitem_relation_type_select":"URI"}}]},"item_6_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_6_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03871193","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00198721","subitem_source_identifier_type":"NCID"}]},"item_6_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"塚越, 美枝子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石原, 力"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石井, 英昭"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"伊古田, 勇人"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山口, 由美子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"柏原, 賢治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"城下, 尚"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村上, 正巳"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-07"}],"displaytype":"detail","filename":"M_murakami_47-48.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"M_murakami_47-48.pdf","url":"https://gunma-u.repo.nii.ac.jp/record/4745/files/M_murakami_47-48.pdf"},"version_id":"b0798c2b-e807-4549-a750-782da5ad222b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Acanthamoeba","subitem_subject_scheme":"Other"},{"subitem_subject":"Acanthamoeba keratitis","subitem_subject_scheme":"Other"},{"subitem_subject":"Corneal scraping cytology","subitem_subject_scheme":"Other"},{"subitem_subject":"Case report","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アカントアメーバ角膜炎の3例 : 角膜擦過細胞診でのアカントアメーバ原虫の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アカントアメーバ角膜炎の3例 : 角膜擦過細胞診でのアカントアメーバ原虫の同定"},{"subitem_title":"Three cases of acanthamoeba keratitis : Detection of protozoa by ordinal cytological preparation of corneal scrapes","subitem_title_language":"en"}]},"item_type_id":"6","owner":"2","path":["738"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-15"},"publish_date":"2010-07-15","publish_status":"0","recid":"4745","relation_version_is_last":true,"title":["アカントアメーバ角膜炎の3例 : 角膜擦過細胞診でのアカントアメーバ原虫の同定"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-06-19T12:27:29.703534+00:00"}