| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2008-11-18 |
| タイトル |
|
|
タイトル |
Gene Expression Profiles of Prostate Cancer Cell Lines, LNCaP, PC-3 and DU-145, Assessed by cDNA Microarray |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
gene expression profile |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cDNA microarray |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
LNCaP |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
PC-3 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
DU-145 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Okamura, Keigo
Koike, Hidekazu
Matsui, Hiroshi
Suzuki, Kazuhiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Purpose: In the basic research of prostate cancer, prostate cancer cell lines are very valuable tools to\ncharacterize the biology of prostate cancer. Three cell lines including LNCaP, PC-3 and DU-145 are\nfrequently utilized. Characterization of molecular characteristics of them is very important. In the\npresent study, we screened gene expression patterns of human prostate cancer cell lines, LNCaP, PC-3 and\nDU-145, using cDNA microarray. Methods: Total RNA from LNCaP, PC-3 or DU-145 cells was\nreversely transcribed in the presence of Cy3 or Cy5 as a probe. Mixed probes were hybridized to a slide\nglass carrying 557 kinds of cancer-related known genes. Signal intensities of Cy-3 and Cy-5 were\nseparately scanned, and relative signal ratios of Cy3/Cy5 were analyzed. Gene expression levels of\nseveral genes were quantified by real-time PCR. Results: We detected over-expressed or underexpressed\ngenes in each cell line. Among these genes,farnesyldiphosphate farnesyltransferase 1, amphiregulin,\nE-cadherin, urokinase-type plasminogen activator (uPA), and glutathione peroxidase 1 gene\nexpression levels were quantified using quantitative real-time PCR. The relation of the gene expression\npatterns of these genes was consistent with those assessed by real-time PCR. Conclusions: Gene\nexpression profiles of human prostate cancer cells varied among cell lines. These profiles will provide\nuseful information on prostate cancer cell lines in the design of experiments, analysis of experimental\ndata, and so on. |
| 書誌情報 |
The Kitakanto medical journal = 北関東医学
巻 58,
号 4,
p. 363-369,
発行日 2008-11-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1343-2826 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10585677 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
北関東医学会 |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Journal Article |
| 更新日 |
|
|
日付 |
2017-03-27 |
|
日付タイプ |
Created |
58_363.pdf (256.9 kB)
