| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2007-12-20 |
| タイトル |
|
|
タイトル |
Methenamine Silver Stain Impregnates Amyloid-related Components of Senile Plaques in the Alzheimer Brains as Clearly as βprotein Immunostaining |
| 言語 |
|
|
言語 |
eng |
| その他のタイトル |
|
|
その他のタイトル |
アルツハイマー病脳老人斑のアミロイド関連成分をβ蛋白免疫感染と同等に明瞭に染めるメセナミン銀染色 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Senile Plaques |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Methenamine Silver Stain |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
βProtein |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Alzheimer-type Dementia |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Down's Syndrome |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Amyloid |
| 著者 |
Yamaguchi, Haruyasu
Haga, Chie
Hirai, Shunsaku
Nakazato, Yoichi
Kosaka, Kenji
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We developed a new type of methenamine silver (MS) stain for senile plaques (SP) on paraffin-embedded tissue sections. This method is a modification of both Gomori's methenamine silver nitrate, and Jone's periodic acid-methenamine silver (PAM) stains. We omitted chemical preoxidation by either periodic acid in the PAM stain or chromic acid in Gomori's stain, and determined the best condition of silver solution for SP. This resulted in selective staining of SP and amyloid angiopathy, because capillary basement membranes, corpora amylacea, macrophage granules, and nerve cell pigments remained unstained. The argyrophilia of SP was not affected by pretreatment with aldehyde blocking reagents. In comparison with βprotein immunostain, our MS stain showed almost the same staining pattern as that of the βprotein immunostain. Moreover, the argyrophilia of SP was selectively abolished by formic acid pretreatment, which has been shown to destroy β-pleated sheet structure of amyloid, suggesting that our MS stain impregnates amyloid-related components of SP. Our MS stain is a new, rapid, easy, and reliable method, and sensitive enough for routine or screening studies of the SP. |
| 書誌情報 |
群馬大学医療技術短期大学部紀要
巻 10,
p. 121-129,
発行日 1990-03-31
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0389-7540 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00068340 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版者 |
|
|
出版者 |
群馬大学医療技術短期大学部 |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Departmental Bulletin Paper |
| 更新日 |
|
|
日付 |
2017-03-27 |
|
日付タイプ |
Created |
KJ00004436168.pdf (1.3 MB)
